Complete this section for all typing done by DNA based methods. Examples of HLA typing by DNA technology may include: sequence-specific primer (SSP), sequence-specific oligonucleotide probe (SSOP), and sequence-based typing (SBT).

DNA technology can be used to type for a single allele, combinations of alleles (allele strings), or a “generic” allele designation similar to a serologic typing result. For this reason, the number of digits reported, as well as the number of alleles, will vary.

Laboratories may use “ / ” , “ – ” or a combination of numbers and letters on the typing report as a shorthand notation for the results. Transcribe the information onto the form as directly as possible. The letters, called allele codes, will be 1 or more characters in length and represent a combination of possible alleles at a locus. The same allele combination may be reported several different ways (e.g., DRB1*01:01 or 01:02, DRB1*01:01/01:02, DRB1*01:01/02, or DRB1*01:AB).

There will be two alleles reported for each locus, unless the individual is presumed homozygous (i.e., carries two copies of the same allele) at a locus. Transcribe the first allele designation in the first box, and the second allele designation in the second box. If the person is homozygous, leave the second box blank.

Question 2: Was documentation submitted to the CIBMTR (e.g., lab report)?

Indicate if a copy of the HLA typing report is attached. Use the “Add Attachment” feature to attach a copy of the HLA typing report in FormsNet3SM. Attaching a copy of the laboratory report assists in confirming the reporting of HLA typing and reduces the need for later data queries.

Class I

Questions 3-4: Locus A

Indicate whether the allele designations at HLA-A are “known” or “unknown.” If known, report the first A* allele and second A* allele designations in question 4; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 5. If question 3 is “unknown,” then question 25 (“A” antigens defined by serologic typing) is required.

Questions 5-6: Locus B

Indicate whether the allele designations at HLA-B are “known” or “unknown.” If known, report the first B* allele and second B* allele designations in question 6; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 7. If question 5 is “unknown,” then question 28 (“B” antigens defined by serologic typing) is required.

Questions 7-8: Locus C

Indicate whether the allele designations at HLA-C are “known” or “unknown.” If known, report the first C* allele and second C* allele designations in question 8; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 9.

Class II

Questions 9-10: Locus DRB1

Indicate whether the allele designations at HLA-DRB1 are “known” or “unknown.” If known, report the first DRB1* allele and second DRB1* allele designations in question 10; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 11.

Class II (Optional)

Questions 11-12: Locus DRB3

Indicate whether the allele designations at HLA-DRB3 are “known” or “unknown.” If known, report the first DRB3* allele and second DRB3* allele designations in question 12; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 13.

Questions 13-14: Locus DRB4

Indicate whether the allele designations at HLA-DRB4 are “known” or “unknown.” If known, report the first DRB4* allele and second DRB4* allele designations in question 14; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 15.

Questions 15-16: Locus DRB5

Indicate whether the allele designations at HLA-DRB5 are “known” or “unknown.” If known, report the first DRB5* allele and second DRB5* allele designations in question 16; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 17.

Questions 17-18: Locus DQB1

Indicate whether the allele designations at HLA-DQB1 are “known” or “unknown.” If known, report the first DQB1* allele and second DQB1* allele designations in question 18; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 19.

Questions 19-20: Locus DPB1

Indicate whether the allele designations at HLA-DPB1 are “known” or “unknown.” If known, report the first DPB1* allele and second DPB1* allele designations in question 20; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 21.

Questions 21-22: Locus DQA1

Indicate whether the allele designations at HLA-DQA1 are “known” or “unknown.” If known, report the first DQA1* allele and second DQA1* allele designations in question 22; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 23.

Questions 23-24: Locus DPA1

Indicate whether the allele designations at HLA-DPA1 are “known” or “unknown.” If known, report the first DPA1* allele and second DPA1* allele designations in question 24; report a single allele, a string of alleles, or an allele code.

If “unknown,” continue with question 25.

Last modified: Jan 27, 2020

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