Question 47: Was transfection efficiency done? (genetically engineered cells)

Answered for genetically engineered/manipulated cells only. Transfection efficiency is calculated as a percentage of transfected cells from all cells in the sample. There are a number of methods used to determine transfection efficiency including flow cytometry, fluorometry, microscopy, real-time quantitative PCR, etc. Indicate “yes” if transfection efficiency was done. Indicate “no” if transfection efficiency was not done and go to question 51. Indicate “Unknown” if it is unclear whether or not transfection efficiency was performed.

Question 48: Date:

Specify the date (YYYY-MM-DD) when sample was taken for the transfection efficiency testing.

If the exact date is unknown, please view General Instructions, General Guidelines for Completing Forms for more information on reporting partial and unknown dates.

Question 49: Transfection efficiency:

Report the percent transfection efficiency. Round to the nearest whole number.

Question 50: Was transfection efficiency target achieved?

Transfection efficiency target will be defined by the protocol. Indicate “yes” or “no” if the target defined by the protocol was met.

Question 51: Was viability of cells done?

If the viability of the cells was quantified, select “yes” and continue with question 52. If viability of the cells was not done, indicate “no” or if it’s unclear if viability was done, indicate “Unknown” and continue with question 56.

Question 52: Date:

Specify the date (YYYY-MM-DD) when the sample was collected to determine viability.

If the exact date is unknown, please view General Instructions, General Guidelines for Completing Forms for more information on reporting partial and unknown dates.

Question 53: Viability of cells:

Report the percent viability. Round to the nearest whole number.

Question 54-55: Method of testing cell viability:

Indicate the method of testing viability.

7-AAD (7-aminoactinomycinD) and Propidiumiodide are compounds that can stain dead cells but will not cross the membrane of living cells. Cytometric techniques are used to calculate the percentage of viable cells in a sample.

Trypan Blue is a technique where the dead cells become stained when in contact with the compound, but living cells remain impermeable to the dye. Cells are counted under a microscope to determine the percentage of viable cells in a sample.

If both methods of viability testing are performed, report 7-AAD results. If the cell viability was tested using a different method, select “other method” and specify the method in question 55.

Section Updates:

Question Number Date of Change Add/Remove/Modify Description Reasoning (If applicable)
. . . . .
Last modified: Dec 22, 2020

Need more help with this?
Don’t hesitate to contact us here.

Was this helpful?

Yes No
You indicated this topic was not helpful to you ...
Could you please leave a comment telling us why? Thank you!
Thanks for your feedback.