Question 38: Was transfection efficiency done? (genetically engineered cells)

Answered for genetically engineered/manipulated cells only. Transfection efficiency is calculated as a percentage of transfected cells from all cells in the sample. There are a number of methods used to determine transfection efficiency including flow cytometry, fluorometry, microscopy, real-time quantitative PCR, etc.

This information will be available from the cell processing lab that is manufacturing the cellular product, and may not be available in the medical record. Indicate Yes if transfection efficiency was done. If transfection efficiency was not done or it is not known if transfection efficiency was performed, select No or Unknown, respectively and continue with question 42.

Question 39: Date:

Specify the date (YYYY-MM-DD) when sample was taken for the transfection efficiency testing.

If the exact date is unknown, please view General Instructions, General Guidelines for Completing Forms for more information on reporting partial and unknown dates.

Question 40: Transfection efficiency:

Report the percent transfection efficiency. Round to the nearest whole number.

Question 41: Was transfection efficiency target achieved?

Transfection efficiency target will be defined by the protocol. Indicate Yes or No if the target defined by the protocol was met.

Question 42: Was viability of cells done?

Indicate if the viability of cells was done. If the viability of the cells was analyzed, select Yes. If viability of the cells was not done or it is not known if completed, indicate No or Unknown, respectively and continue with question 47.

Question 43: Date:

Specify the date (YYYY-MM-DD) when the sample was analyzed to determine viability, not the date the results are available.

If the exact date is unknown, please view General Instructions, General Guidelines for Completing Forms for more information on reporting partial and unknown dates.

Question 44: Viability of cells:

Report the percent viability. Round to the nearest whole number.

Question 45-46: Method of testing cell viability:

Indicate the method of testing viability.

7-AAD (7-aminoactinomycinD) and Propidium iodide are compounds that can stain dead cells but will not cross the membrane of living cells. Cytometric techniques are used to calculate the percentage of viable cells in a sample.

Trypan Blue is a technique where the dead cells become stained when in contact with the compound, but living cells remain impermeable to the dye. Cells are counted under a microscope to determine the percentage of viable cells in a sample.

If both methods of viability testing are performed, report 7-AAD results. If the cell viability was tested using a different method, select Other method and specify the method in question 46.

Section Updates:

Question Number Date of Change Add/Remove/Modify Description Reasoning (If applicable)
. . . . .
Last modified: Jul 23, 2021

Need more help with this?
Don’t hesitate to contact us here.

Was this helpful?

Yes No
You indicated this topic was not helpful to you ...
Could you please leave a comment telling us why? Thank you!
Thanks for your feedback.